RT-PCR analyses of

IGnT gene expression in the RNA samples purified from whole blood cells and reticulocytes and from lens-epithelium cells. Pure reticulocytes were isolated from whole blood through positive isolation of cells expressing the transferrin receptor (CD71) by immunomagnetic separation, as described in “Patients, materials, and methods.” Total RNA from whole blood cells (the buffy coat layer) and reticulocytes (A) and lens-epithelium cells (B) was prepared, and RT-PCR analysis for the IGnTA, IGnTB, andIGnTC transcripts was conducted as described in “Patients, materials, and methods” and the legend of Figure 3.