Increased angiogenesis and inflammation in cutaneous DTH reactions elicited in PlGF-2 transgenic mice.

Increased edema formation and inflammatory infiltration in the skin of PlGF-2 transgenic mice (B) at 24 hours after antigen challenge as compared with wild-type mice (A). Hematoxylin and eosin stains. CD31 stains demonstrated enhanced angiogenesis at 24 hours after challenge in PlGF-2 transgenic mice (D) as compared with wild-type mice (C). (E-H) In situ hybridization confirmed high levels of human PlGF-2 mRNA expression in transgenic epidermal keratinocytes at 24 hours after challenge (E-F), whereas no hybridization signal for human PlGF-2 was detected in wild-type mice (G-H). Bright-field (E,G) and dark-field (F,H) micrographs. Scale bars = 50 μm.