IFN-α production by mouse blood pre-DCs.

Sorted populations of mouse blood pre-DCs were stimulated in culture with CpG and IL-3 or IL-3 alone. Supernatants were harvested after 36 hours, then analyzed by bioassay for type 1 IFN production. Data are from one experiment; replicate samples produced identical results. Similar results were obtained in 3 experiments. Results were also confirmed by ELISA for IFN-α.