Fig. 4.
Fig. 4. Surface phenotype of mouse blood pre-DCs after in vitro maturation. / Mouse blood pre-DC populations were incubated with their respective optimal stimuli, as described in “Results.” After 36-hour culture, the cells were recovered and stained with antibodies to CD11c, CD4, CD8, and MHC class 2. Panel A shows the surface staining of CD11cintCD45RA− mouse pre-DCs after incubation with GM-CSF and TNF-α. Panel B shows the surface staining of CD11cloCD45RAhi cells after incubation with GM-CSF, IL-3, and CpG. In both panels, the unfilled line within the MHC class 2 histogram gives the background fluorescence with only the MHC class 2 stain omitted. Data are from one experiment, representative of 5 to 10 separate experiments, each of which examined the pre-DCs obtained from the pooled blood of more than 20 mice.

Surface phenotype of mouse blood pre-DCs after in vitro maturation.

Mouse blood pre-DC populations were incubated with their respective optimal stimuli, as described in “Results.” After 36-hour culture, the cells were recovered and stained with antibodies to CD11c, CD4, CD8, and MHC class 2. Panel A shows the surface staining of CD11cintCD45RA− mouse pre-DCs after incubation with GM-CSF and TNF-α. Panel B shows the surface staining of CD11cloCD45RAhi cells after incubation with GM-CSF, IL-3, and CpG. In both panels, the unfilled line within the MHC class 2 histogram gives the background fluorescence with only the MHC class 2 stain omitted. Data are from one experiment, representative of 5 to 10 separate experiments, each of which examined the pre-DCs obtained from the pooled blood of more than 20 mice.

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