Effect of Bcr-Abl expression on G-CSF receptor expression in 32D_Bcr-Ablwt cells.

Expression of Bcr-Abl does not diminish G-CSF receptor expression in 32D_Bcr-Ablwt cells. The 32Dcl3 or 32D_Bcr-Ablwt cells grown in the presence of IL-3 were subjected to Western blot (A), real-time PCR (B), confocal microscopy (C, top row), or FACS analysis (C, bottom row) as described in “Materials and methods.” The myeloid, G-CSFR–deficient cell line L-GM was used as a negative control where indicated. Equal expression of G-CSFR in the presence or absence of Bcr-Abl expression was seen in 2 independent subclones of 32D_Bcr-Ablwt.