Fig. 3.
Correlation of increase in O2−with cell viability (%) in CLL cells treated with 2-ME in vitro.
Primary leukemia cells isolated from 50 patients with CLL were treated with 10 (○) or 30 (●) μM 2-ME for 24 hours. The O2− levels and cell viability were determined by flow cytometry analysis and MTT assay as described in “Materials and methods.” Linear regression analysis indicates that 2-ME–induced increase of cellular O2− is significantly correlated with the loss of cell viability (P < .001).
