Fig. 5.
Fig. 5. Expression of CD41 and CD45 in hematopoietic progenitors during embryonic and fetal hematopoiesis in vitro and in vivo. / (A) FACS sorting of d6 EBs (left), E9.5 yolk sac (middle), and E14.5 fetal liver (right). (B) Percentage of definitive CFU-C in EB subpopulations expressing CD41 and/or CD45, yolk sac, and fetal liver (average of 3 independent experiments). Pale blue indicates CD45−CD41−; yellow, CD45−CD41+; dark red, CD45+CD41+; and medium blue, CD45+CD41−. (C-D) May-Grünwald-Giemsa staining of individual CFU-mix colonies from CD45−CD41+ (C) and CD45+CD41+ (D) subpopulations of E9.5 yolk sac. Arrows demonstrate the presence of definitive erythroid cells (ery), megakaryocytes (meg), macrophages (mac), and mast cells (mast) in individual colonies from both populations. Original magnifications C-D, × 200.

Expression of CD41 and CD45 in hematopoietic progenitors during embryonic and fetal hematopoiesis in vitro and in vivo.

(A) FACS sorting of d6 EBs (left), E9.5 yolk sac (middle), and E14.5 fetal liver (right). (B) Percentage of definitive CFU-C in EB subpopulations expressing CD41 and/or CD45, yolk sac, and fetal liver (average of 3 independent experiments). Pale blue indicates CD45−CD41−; yellow, CD45−CD41+; dark red, CD45+CD41+; and medium blue, CD45+CD41−. (C-D) May-Grünwald-Giemsa staining of individual CFU-mix colonies from CD45−CD41+ (C) and CD45+CD41+ (D) subpopulations of E9.5 yolk sac. Arrows demonstrate the presence of definitive erythroid cells (ery), megakaryocytes (meg), macrophages (mac), and mast cells (mast) in individual colonies from both populations. Original magnifications C-D, × 200.

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