American Society of Hematology

Fig. 3.

$Fig. 3. Hematopoietic and endothelial potential of CD41+ and CD41− fractions in embryoid bodies. / Embryoid bodies were differentiated for 6 to 7 days, separated into single-cell suspension, and sorted based on surface expression of CD41. FACS-sorted cells were subjected to May-Grünwald-Giemsa staining and culture in hematopoietic or endothelial cell growth conditions. (A) May-Grünwald-Giemsa staining of CD41+ cells. (B) May-Grünwald-Giemsa staining of CD41− cells. (C) Methylcellulose colony assay of CD41+ cells. (D) Methylcellulose colony assay of CD41− cells. (E) Endothelial cell culture of CD41+ cells. (F) Endothelial cell culture of CD41− cells. (G) Isotype control and (H) Flk/PECAM staining of endothelial cell cultures from CD41− cells. Original magnifications A-B, × 400; C-D, × 40; E-F, × 100.$

Hematopoietic and endothelial potential of CD41⁺ and CD41⁻ fractions in embryoid bodies.

Embryoid bodies were differentiated for 6 to 7 days, separated into single-cell suspension, and sorted based on surface expression of CD41. FACS-sorted cells were subjected to May-Grünwald-Giemsa staining and culture in hematopoietic or endothelial cell growth conditions. (A) May-Grünwald-Giemsa staining of CD41⁺ cells. (B) May-Grünwald-Giemsa staining of CD41⁻ cells. (C) Methylcellulose colony assay of CD41⁺ cells. (D) Methylcellulose colony assay of CD41⁻ cells. (E) Endothelial cell culture of CD41⁺ cells. (F) Endothelial cell culture of CD41⁻ cells. (G) Isotype control and (H) Flk/PECAM staining of endothelial cell cultures from CD41⁻ cells. Original magnifications A-B, × 400; C-D, × 40; E-F, × 100.

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