Experimental model to assess resistance against MiHA-mismatched allogeneic bone marrow grafts.

Normal or cytotoxically deficient mice were primed with donor cells (“Materials and methods”) at least 3 weeks prior to irradiation (day −1) and BM transplantation (day 0). Recipients received varying doses of donor or syngeneic BM-TCD and resistance was analyzed by CFU activity in recipient spleens (day 5) and donor (ie, C3H.SW) chimerism by staining with Ly9.1 mAb.