Fig. 2.
![Fig. 2. Effect of VX-745 on paracrine MM cell growth in the BM. / (A) MM.1S (●), RPMI8226 (▪), and U266 (▴) (3 × 104) cells were cultured in the presence of VX-745 (0.06 μM-20 μM) for 48 hours, and cell viability was assessed by MTT assay. (B) MM.1S cells were cultured in BMSC-coated 96-well plates for 48 hours, in the absence (■) or presence of 0.2 μM (▤), 1 μM (▧), and 5 μM (▪) VX-745. DNA synthesis was assessed by [3H]TdR uptake. (C) BMSCs were cultured with 3 × 104 MM.1S cells in the absence (■) or presence of 0.2 μM (▤), 1 μM (▧), and 5 μM (▪) VX-745 for 24 hours. IL-6 in culture supernatants was measured by ELISA.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/2/10.1182_blood-2002-06-1874/4/h80233671002.jpeg?Expires=1722615311&Signature=kO69WDqUqRFtDFYMgV1kFdwHJHboUiR1GsATcy6nXTYxa0TflN-JacbfLDIebreNS7QGwZ4EE9oumyjn-~LpWVQSMZbBQm4ZGb-JfvTRU7Nso4ps72I7J0hCZVZolp5z8Uy3MoMJj2muOAf7OVEQCZMePPCMsg9k27GbCl85WntC86UU0Ac7tHsyTVLTMyM6YioJLxiQFR5MBb6lEL4wPXJ2N1Vd61lmdL09t1oH8F4opIq7n7cBRCnJ7usCyy4sakul2zuAk2xa50vuew7hLQpSpQazhTrtIQomlZgJ9OpTPefP7YlejJD8WE1S~7-yFfTZaAS5~kaZxUZ8dSUNBg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of VX-745 on paracrine MM cell growth in the BM.
(A) MM.1S (●), RPMI8226 (▪), and U266 (▴) (3 × 104) cells were cultured in the presence of VX-745 (0.06 μM-20 μM) for 48 hours, and cell viability was assessed by MTT assay. (B) MM.1S cells were cultured in BMSC-coated 96-well plates for 48 hours, in the absence (■) or presence of 0.2 μM (▤), 1 μM (▧), and 5 μM (▪) VX-745. DNA synthesis was assessed by [3H]TdR uptake. (C) BMSCs were cultured with 3 × 104 MM.1S cells in the absence (■) or presence of 0.2 μM (▤), 1 μM (▧), and 5 μM (▪) VX-745 for 24 hours. IL-6 in culture supernatants was measured by ELISA.
