Human cytokeratin 19 expression in the livers of injured mice that underwent human stem cell transplantation and were treated with HGF.
When NOD/SCID/β2M-null mice received transplants of human stem cells (CB- or BM-derived CD34+ cells) and were treated with CCl4 and HGF MSCs, cells expressing mRNA for the human cholangiocyte marker cytokeratin 19 (CK19) were found in the mouse liver. In the injured, HGF-treated chimeric mice, 2 bands were observed. The first was murine CK19, which comigrated with the band from the control mice that did not undergo transplantation. The second band was a human-specific band of the expected size, which comigrated with the CK19 band from a human liver control. No human CK19 was detected in the murine control liver, or in freshly isolated CD34+ hematopoietic progenitors. The first lane shows molecular weights.
