Quantitative real-time RT-PCR analysis of platelet transcripts.

Real-time RT-PCR was completed by using purified platelet RNA and oligonucleotide primer pairs specifically designed using Primer3 software to generate similarly-sized (∼200-bp) PCR products, optimized to the same annealing temperature. In graph, (■) represents IIb, (▪) represents IIIa, (▵) represents PAR1, (▴) represents 16S rRNA, (▿) represents NADH2, (▾) represents thymosin, (⋄) represents clusterin, (♦) represents neurogranin, and (●) represents TCRβ. Curves are representative of one complete set of experiments (repeated twice), and line plots reflect average determinations from 3 wells performed in parallel with SEM less than 1% for all data points.