Influence of G-CSF on granulocyte survival.

Granulocytes from mice of the indicated genotypes were isolated from (A, C) bone marrow or (B) the peritoneal cavity after injection with casein. Granulocytes were cultured in the absence or presence of rhG-CSF (100 U/mL). Cell survival was assessed after 72 hours (A; bone marrow–derived granulocytes) or 24 hours (B; mobilized peritoneal granulocytes) or at time points (C; bone marrow) by staining with propidium iodide plus or minus annexin V staining and flow cytometric analysis. Both methods gave comparable results, and data were therefore pooled. Bars represent means ± SDs of 3 to 6 independent experiments performed in duplicate on 4 to 8 animals of each genotype.