SU5614 induces apoptosis and cell cycle arrest in Ba/F3 cells transformed by the FLT3ITD-NPOS mutant.

(A,C) Ba/F3 cells transformed by FLT3ITD-NPOS were stained with annexin V–PE and 7-AAD and analyzed by flow cytometry. Representative dot plots from 1 of 3 independently performed experiments are shown. Values represent the means ± SDs of 3 independent experiments. (B) DNA fragmentation identified by DNA laddering on agarose gel electrophoresis in Ba/F3 FLT3ITD-NPOS. Cells were incubated with SU5614 in the presence or absence of recombinant murine IL-3 (10 ng/mL) for the indicated time intervals. gDNA was analyzed in a 2% agarose gel. (D) Cell cycle analyses were performed by determination of the DNA content with PI. Cells were stained with PI and cell nuclei were analyzed by flow cytometry. *P < .05; **P < .01; ***P < .001 according to Student t test comparing the percentage of cells in G₀/G₁, S, and G₂/M phases in untreated versus inhibitor-treated samples.