LPS induction of IL-6 and MCP-1 mRNA expression in the kidneys of Egr-1+/+ and Egr-1−/− mice.
Egr-1+/+ and Egr-1−/− mice were either untreated (3 mice) or injected with LPS (4 mice per group) for either 3 or 8 hours. Total RNA was isolated from the kidney. (A) Various cytokine mRNAs were measured by RPA using the mouse cytokine multiprobe template set mCK-3b (Pharmingen). This probe set contains (left lane of each gel) TNFβ, LTβ, TNFα, IL-6, IFNγ, IFNβ, TGFβ1, TGFβ2, TGFβ3, and MIF. LPS induced IL-6 mRNA expression, whereas the constitutive expression of MIF was not affected by LPS. Levels of IL-6 mRNA normalized to G3PDH are shown below the gel (mean ± SD). (B) Various chemokine mRNAs were measured by RPA using the mouse chemokine multiprobe template set mCK-5 (Pharmingen). This probe set (left lane of each gel) contains Ltn, RANTES, Eotaxin, MIP-1β, MIP-1α, MIP-2, IP-10, MCP-1, and TCA-3. LPS induced RANTES, MIP-1β, MIP-2, MCP-1, and TCA-3 mRNA expression. Levels of MCP-1 mRNA normalized to G3PDH are shown below the gel (mean ± SD). The asterisks indicate decreases that were statistically significant (P < .05).
