Retroviral construct, stem cells, and transduction.

(A) Schematic representation of the retroviral vector, pMY/HES-1IG, encoding HES-1 linked by internal ribosome entry site (IRES) to a cDNA encoding EGFP. (B) Flow cytometric analysis of murine hematopoietic stem cells in adult bone marrow and fetal liver. Staining profile of Sca-1 versus c-Kit on lineage-negative populations in bone marrow (left) and fetal liver (right) cells. (C) Flow cytometric analysis for GFP in infected c-Kit⁺ Sca-1⁺ Lin⁻(KSL) cells of bone marrow (top) and fetal liver (bottom). Efficiency of infection of these populations is indicated.