Fig. 3.
B cells continued to divide under the conditions used for transduction.
B cells were activated for 4 days in primary culture, then isolated and cultured for 15 hours in medium/cytokines, as for the transduction in Figure 2. Shown are profiles for cellular DNA content obtained at the end of the 15-hour incubation, performed either in the absence (Control) or presence of mitosis inhibitor (Nocodazole, 50 ng/mL). Percentages of B cells in late S/G2/M are indicated. Peaks of cells with hypodiploid DNA content represent apoptotic cells.32
