American Society of Hematology

Fig. 2.

$Fig. 2. Comparison of HIV vectors expressing GFP under the control of PGK, EF1-α, CAG, or CMV promoters and of an MLV vector with the CMV promoter. / B cells were transduced and analyzed as in Figure 1B, in parallel with the indicated vectors (MOI, 10). In the FACS 2D plots, the y-axis represents cells stained by anti-CD19 or isotype control. Mean fluorescence of nontransduced cells or of the GFP+ fraction of transduced cells (MF) and percentages of GFP+ among CD19+ cells (T) are indicated. CD19low cells are usually more differentiated, plasmocytic cells in this culture system.$

Comparison of HIV vectors expressing GFP under the control of PGK, EF1-α, CAG, or CMV promoters and of an MLV vector with the CMV promoter.

B cells were transduced and analyzed as in Figure 1B, in parallel with the indicated vectors (MOI, 10). In the FACS 2D plots, the y-axis represents cells stained by anti-CD19 or isotype control. Mean fluorescence of nontransduced cells or of the GFP⁺ fraction of transduced cells (MF) and percentages of GFP⁺ among CD19⁺ cells (T) are indicated. CD19^low cells are usually more differentiated, plasmocytic cells in this culture system.

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