Fig. 5.
Fig. 5. Functional analysis of mutated regulatory sequences in theCBS −1b basal promoter and upstream regions. / CMK cells were transfected with the wild-type and mutant CBS−1b promoter reporter gene constructs spanning the basal (positions −3792 to −3667) and upstream regions (positions −4046 to −3792) of the CBS −1b promoter, as described in “Materials and methods.” The oligonucleotides used for preparing the mutant CBS −1b promoter GC-g, GC-f, GT-d, USF-1, and NF-Y constructs were previously described.1617 Luciferase activities of the mutant constructs were normalized toRenilla luciferase activities and compared with that of the −4046/−3565 wild-type construct. Data are presented as the means ± SE from 3 independent experiments performed in duplicate.

Functional analysis of mutated regulatory sequences in theCBS −1b basal promoter and upstream regions.

CMK cells were transfected with the wild-type and mutant CBS−1b promoter reporter gene constructs spanning the basal (positions −3792 to −3667) and upstream regions (positions −4046 to −3792) of the CBS −1b promoter, as described in “Materials and methods.” The oligonucleotides used for preparing the mutant CBS −1b promoter GC-g, GC-f, GT-d, USF-1, and NF-Y constructs were previously described.16,17 Luciferase activities of the mutant constructs were normalized toRenilla luciferase activities and compared with that of the −4046/−3565 wild-type construct. Data are presented as the means ± SE from 3 independent experiments performed in duplicate.

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