Fig. 3.
Fig. 3. EMSAs with CMK and CMS nuclear extracts spanning the upstream and basal regions of the CBS −1b promoter. / Gel shift assays were performed with CMK and CMS nuclear extracts and the 32P-labeled FPA, FPB, FPC, FPD oligonucleotide probes (designated A-D, respectively), spanning upstream regions of theCBS promoter (positions −4046 to −3792), andCBS −1b basal promoter probes (−3766/−3725 and −3724/−3682; designated E and F, respectively). Major DNA/protein complexes were previously identified in the HepG2 cell line (control) and are indicated.1617 NS indicates a nonspecific complex.

EMSAs with CMK and CMS nuclear extracts spanning the upstream and basal regions of the CBS −1b promoter.

Gel shift assays were performed with CMK and CMS nuclear extracts and the 32P-labeled FPA, FPB, FPC, FPD oligonucleotide probes (designated A-D, respectively), spanning upstream regions of theCBS promoter (positions −4046 to −3792), andCBS −1b basal promoter probes (−3766/−3725 and −3724/−3682; designated E and F, respectively). Major DNA/protein complexes were previously identified in the HepG2 cell line (control) and are indicated.16,17 NS indicates a nonspecific complex.

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