Expression of wild-type or mutated murine PSGL-1 on transfected CHO cells coexpressing FTVII.

The cells were permanently transfected with an empty expression vector (control) or with an expression vector encoding wild-type murine PSGL-1 or the indicated mutant PSGL-1 construct. Staining with antimurine PSGL-1 mAb 2PH1 was performed as in Figure 2. The data are representative of at least 5 experiments.