Schematic diagram of murine PSGL-1 constructs.

(A) Diagram of the domains in human and murine PSGL-1. (B) Alignment of the mature N-terminal sequences of human and murine PSGL-1. A one-residue gap in the human sequence was introduced to align the putative O-glycosylated threonine and the conserved N-glycosylation site with those in the murine sequence. (C) Sequences of the amino acid substitutions in the full-length murine PSGL-1 constructs.