WASP is expressed in mature T cells from RAG-2 recipients of transplanted retrovirally transduced WKO-HSCs.

(A) Schemes of retroviral constructs used in this study. (B) Western analysis of T cells from RAG-2 recipients of transplanted (1) WT HSCs transduced with CMMP-GFP; (2) WKO HSCs transduced with CMMP-GFP; (3) WKO HSCs transduced with CMMP-WASP. WASP is expressed (arrow) in T cells in RAG-2 recipient mice that received transplants of either WT HSCs or WASP HSCs transduced with CMMP-WASP, but not CMMP-GFP. The lower panel depicts loading controls (actin staining).