Fig. 5.
![Fig. 5. SDF-1–induced calcium mobilization. / Stably transfected HEK/CD4.403 cells expressing wild-type and mutated CXCR4 receptors were loaded with Fluo-3-AM. SDF-1–induced calcium flux was measured as described in “Materials and methods” in the presence (○) or absence (●) of PTX preincubated overnight at 100 ng/mL. Representative curves of [Ca2+]i changes after stimulation with SDF-1α and ionomycin (I) from 1 to 4 experiments are shown.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/2/10.1182_blood-2002-03-0978/4/h80233648005.jpeg?Expires=1722710479&Signature=S41CR-GV7FjreLAnXtVzM5e0T1qBfh6ryzX0o9MsQSuRGbNvnNW3SY1Ih2mmPa0Suepo4NIpuxPiGP~us6GHf9O0~JuwOJm511EuNJaJslZyN9q0n9pFomcZTeQVRvYkNjEU15p0t54nWTVciSiusdQGLwls~EVfh8uc1ZEzEiBNDgTbqtSXxsKGx8MzpTQfJuenyFl6NJJun5h6ao-5KJWWVHVLVCtt5mkYDXNJoLlhd~Soznajd2fP1VmECzEJy5f6~MCR4zDVGtOIKbRtkk81RZantX~HRhYDBf-vngA8MhHag2XqFy8W0cVsEhqnaWZhkfvIH5oZXgq0ik-WoQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
SDF-1–induced calcium mobilization.
Stably transfected HEK/CD4.403 cells expressing wild-type and mutated CXCR4 receptors were loaded with Fluo-3-AM. SDF-1–induced calcium flux was measured as described in “Materials and methods” in the presence (○) or absence (●) of PTX preincubated overnight at 100 ng/mL. Representative curves of [Ca2+]i changes after stimulation with SDF-1α and ionomycin (I) from 1 to 4 experiments are shown.
