Expression of wild-type and mutated CXCR4 molecules at the surface of the HEK/CD4.403 cell line.

Cells were incubated with medium containing the anti-CXCR4 mAb at 10 μg/mL. Bound mAb was detected with a FITC-labeled antimouse immunoglobulin. The white histogram represents binding of anti-CXCR4 mAb to HEK/CD4.403 cells, and black histograms to the different CXCR4 molecules analyzed. The fluorescence intensity was recorded in log mode on an EPICS XL4 cytofluorometer. Data representative of 1 to 5 independent experiments are shown.