DXM, IL-10, or Th2 cytokine treatment of THP-1 cells induces the association of GILZ with p65.

(A-B) THP-1 cells were cultured for 4 hours alone or with DXM, IL-4, IL-10, or IL-13. Cellular protein was immunoprecipitated with anti-p65 antiserum. Immunoblotting was performed with anti-p65 (A) or anti-GILZ (B) antibody. HC: heavy chain of the antibody used in the assay. (C) THP-1 cells were transfected either with the pcDNA3 control vector alone or with the NFkB-luciferase vector, with or without the GILZ gene–encoding vector. Luciferase activity was determined 4 hours after stimulation with LPS. Results are from 1 experiment typical of 3. Luciferase activity for cells transfected with pcDNA3 alone was < 10 units.