American Society of Hematology

Fig. 7.

$Fig. 7. GILZ inhibits CD80 and CD86 expression. / (A) THP-1 cells were cultured with and without DXM or IL-10. (B) THP-1 cells were transfected with the control (pcDNA3) vector or with the GILZ-encoding vector. CD80 (left panels) and CD86 (right panels) expression was determined by flow cytometry at the end of culture. The dashed curves correspond to the staining obtained with the control mAb. Labeling with anti-CD80 or anti-CD86 antibody is shown by the open histograms and solid histograms for cells cultured without and with IFNγ, respectively. Results shown are from 1 experiment of 4. In the upper right-hand corner of each histogram is the mean fraction (%) of cells in which IFNγ stimulated CD80 or CD86 expression in these 4 experiments.$

GILZ inhibits CD80 and CD86 expression.

(A) THP-1 cells were cultured with and without DXM or IL-10. (B) THP-1 cells were transfected with the control (pcDNA3) vector or with the GILZ-encoding vector. CD80 (left panels) and CD86 (right panels) expression was determined by flow cytometry at the end of culture. The dashed curves correspond to the staining obtained with the control mAb. Labeling with anti-CD80 or anti-CD86 antibody is shown by the open histograms and solid histograms for cells cultured without and with IFNγ, respectively. Results shown are from 1 experiment of 4. In the upper right-hand corner of each histogram is the mean fraction (%) of cells in which IFNγ stimulated CD80 or CD86 expression in these 4 experiments.

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