In vitro induction of GILZ by GCs.

Production of GILZ by human monocytes cultured alone or with DXM was studied by RT-PCR (A-B) and by Western blotting (C). (A) GILZ RT-PCR, 3 experiments. (B) Optical density of products amplified from GILZ mRNA (mean ± SEM) in these 3 experiments. (C) GILZ protein in cells cultured without (lane 1) or with (lane 2) DXM. Results from 1 experiment representative of 2 are shown.