Mapping of the ELL interaction domain within EAF2.

293 cells were cotransfected with GAL4-tagged fragments of EAF2 and FLAG-tagged ELL. The left panel demonstrates expression of full-length ELL with the FLAG antibody for each of the transfections, and the middle panel the expression of the GAL4-EAF2 fragments with the GAL4 antibody. The cell lysates were immunoprecipitated with the FLAG antibody and probed with the GAL4 antibody. EAF2 amino acids 17-104 coprecipitated with ELL, but EAF2 17-100 and 20-104 did not bind to ELL.