Fig. 1.
Fig. 1. Effect of ATRA versus TGF-β1 on expression of Smad mRNAs. / Expression of Smad mRNAs is not changed following induction of differentiation of HL-60 cells to monocytes or granulocytes by 10 nM ATRA or 10 ng/mL TGF-β1. Human Smad-specific primer pairs were selected from the corresponding cDNA sequence information obtained from the National Institutes of Health (NIH) database as indicated in “Materials and methods.” Primer pairs were used to amplify Smad-specific fragments from reverse-transcribed total RNA isolated from the indicated samples as template. In all cases, 1 μg total RNA, quantified by spectrophotometry and agarose gel analysis, was used for reverse transcription. Smad2, Smad3, Smad4, Smad7, and GAPDH (as an internal control) were amplified by PCR and analyzed on an agarose gel.

Effect of ATRA versus TGF-β1 on expression of Smad mRNAs.

Expression of Smad mRNAs is not changed following induction of differentiation of HL-60 cells to monocytes or granulocytes by 10 nM ATRA or 10 ng/mL TGF-β1. Human Smad-specific primer pairs were selected from the corresponding cDNA sequence information obtained from the National Institutes of Health (NIH) database as indicated in “Materials and methods.” Primer pairs were used to amplify Smad-specific fragments from reverse-transcribed total RNA isolated from the indicated samples as template. In all cases, 1 μg total RNA, quantified by spectrophotometry and agarose gel analysis, was used for reverse transcription. Smad2, Smad3, Smad4, Smad7, and GAPDH (as an internal control) were amplified by PCR and analyzed on an agarose gel.

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