Effect of the Ras signaling inhibitor U0126 onCD11c promoter activity in hairy cells.

The expression construct p11Wt was transfected into the hairy cell lines Mo, EH, and HK in parallel with the empty vector pATLuc. Transfected cells were then either left untreated or treated for 24 hours with 8 μM U0126. The level of luciferase reporter gene activity directed by p11Wt above that conferred by pATLuc in the absence of treatment with U0126 and after correction for transfection efficiency is assigned a value of 100% (UT). The level of activity directed by p11Wt in parallel transfections in the presence of U0126 is expressed as a percentage of this value. Each bar represents the mean ± the standard deviation of 3 independent experiments. The Student t test was used to determine the probability values for the changes in promoter activity caused by treatment with U0126. These values for Mo, HK, and EH cells wereP = .028, P = .105, andP = .001, respectively.