Expression of activated Ras induces endogenous CD13/APN expression in endothelial cells and fibroblasts.

Matched NIH-3T3 fibroblast (lanes 1-3) or murine endothelial MS1 (lanes 4-5) cell lines stably transfected with either empty vector control plasmids (lane 2: NIH-3T3 + vector, and lane 4: MS1) or activated Ras-V12 expression constructs (lane 3: NIH-3T3 + Ras V12, and lane 5: SVR) were assessed by semiquantitative RT-PCR for CD13 or control β-actin levels. Identity of the RT-PCR products was verified by Southern blot analysis probed with CD13/APN cDNA (bottom panel). Lane 1 shows untransfected parental NIH-3T3 fibroblasts.