Kinetics of HTLV Env receptor expression following TCR engagement.

Freshly isolated CD4⁺ lymphocytes were activated with immobilized αCD3 and αCD28 mAbs and expression of the HTLV Env receptor was followed after 2, 4, 8, 24, 72, and 216 hours of culture using H_RBD. Cells were simultaneously evaluated for expression of the CD25 (IL-2Rα) and CD69 activation markers using PE-conjugated mAbs. Staining profiles are shown and filled histograms depict staining with an FITC-conjugated secondary mAb alone or with isotype-matched PE-conjugated control mAbs. Results are representative of data obtained in 4 independent experiments.