Effect of oligonucleotides on LYN expression and growth of K562 and K562-R cells.

(A) K562 and K562-R cells were incubated with sense of antisense oligonucleotides as described in “Materials and methods.” After 72 hours, cells were collected and washed, and LYN expression was determined by immunoblotting equal protein aliquots. Actin was probed on the same membrane to determine protein equivalence for each sample. (B) K562 or K562-R cells were incubated with media alone (control) or with sense or antisense oligonucleotides (72 hours total) before cell growth was estimated by MTT assay. Each data point represents the average ± SEM of 3 determinations. Untreated (control) cell survival was set at 100%.