The effect of coagulation factors VIIa, X, and II on adhesion of platelets from patients with type 1 GT to the subendothelial matrix of PMA-stimulated endothelial cells under flow conditions.

Washed platelets and red cells (200 000 platelets/μL; hematocrit 40%) were perfused over PMA-ECM for 5 minutes at 1600 s⁻¹in absence (A) or presence (B) of a thrombin-generating system (1.2 μg/mL rFVIIa, 10 μg factor X, 20 ng/mL prothrombin, 3 mM CaCl₂). Shown is a typical example from one patient with GT. Original magnification × 400 for panels A-B.