Enforced expression of

CUL-4A alters the expression of p130 and c-myc after induction of granulocytic differentiation. The cell lines generated from PLB-985 cells transfected with pEF-PGKpac (empty vector) or with pEFpac-CUL-4A-HA (CUL-4A-HA2) were induced to differentiate into granulocytes for 0 to 6 days and total protein was isolated from a sample taken at each time point. Protein (10 μg) from each sample was electrophoresed, and the amount of the indicated protein was determined by immunoblot and normalized with respect to the amount of actin. Representative results of 3 independent experiments are shown.