Histologic and immunohistologic results of the renal biopsy sample.

(A) A glomerulus of the percutaneous biopsy specimen showing mesangial proliferative nephritis with a fibrotic crescent. Periodic acid-Schiff (PAS) stain; original magnification, × 200. (B) The interstice was massively infiltrated with mononuclear cells shown by light microscopy. Hematoxylin-eosin (HE) stain; original magnification, × 100. (C) Immunofluorescence results showing a glomerulus of the percutaneous biopsy specimen with severe IgA deposition at the mesangial region and along the glomerular loops. Original magnification × 200. (D) Immunohistologic results with the anti-CD8 antibody. Original magnification, × 200. (E) FISH study using a PE-conjugated X chromosome–specific probe and an FITC-conjugated Y chromosome–specific probe. Infiltrated cells had 2 red spots, indicating that infiltrated mononuclear cells in the interstice were donor-derived (female) cells. Original magnification, × 100. (F) Immunofluorescence micrograph of a glomerulus with anti-OMHP antibody. OMHP antigen deposition was mainly in mesangial areas (arrows). Original magnification, × 200.