Intracellular eotaxin expression in tumor cell suspensions.

(A) Analysis gated on high forward- and side-scatter cell population (R1), which corresponds to CD30⁺ tumor cells (patient no. 8). Data shown are representative of 3 experiments. (B) Intracellular flow cytometric staining of tumor cell suspensions for CCR3 ligand eotaxin/CCL11, after stimulation for 4 hours with phorbol myristate acetate (PMA) and ionomycin demonstrates expression of eotaxin in tumor cells