Induction of tolerance to α-gal epitopes by WT lymphocytes.
(A) Production of anti-Gal IgG in KO mice immunized 4 times with pig kidney membranes (PKM) (●) and in KO mice that received 20 × 106 (○) or 2 × 106 (▵) WT lymphocytes prior to the 4 PKM immunizations. Data are from 8 mice in the first group, 8 in the second group, and 4 in the third group. Mean of data in the (●) group is presented as (■). In the (○) and (▵) groups no mean is presented since all mice displayed a complete lack of anti-Gal response. Statistical analysis by t tests indicated P < .05 between tolerized and control mice in all points. (B) ELISPOT analysis of anti-Gal secretion by spleen lymphocytes obtained from KO mice receiving 2 × 106 WT lymphocytes and immunized 4 times with PKM (○) (mean presented as ⋄), or from control mice undergoing the same PKM immunization (●). Mean presented as ⧫. Data are from 4 mice in each group. Statistical analysis by t tests indicated P < .05 between tolerized and control mice in all points.
