Chemotaxis of TH subsets to CCR10 and CCR4 ligands.
Peripheral blood lymphocytes were attracted to optimal concentrations of CTACK or TARC through 5-μm pores, and 5-color immunostaining was performed. Migration is expressed as percent of input (lower scale) and percent of response to optimal concentration of SDF-1α for each subset (upper scale). Background migration for each subset (ranging from 0.5%-3.0%) has been subtracted. Mean and SEM are shown for CD4+ subsets from 3 healthy donors. For each donor, migration was performed in duplicate for medium alone, SDF-1α, CTACK, and TARC. The difference in CTACK responsiveness between CD27+/CCR7+ and CD27−/CCR7− cutaneous TH cells was significant using the Mann-Whitney rank-sum test (P < .05). The difference in TARC responsiveness between these same 2 populations was not significant.
