Fig. 7.
LMP1 expression in fibroblasts and LCLs.
Fibroblasts were harvested 24 hours after transduction with Ad_LMP1 and Ad_ΔLMP1; 2 × 103 cells per lane of fibroblasts and 2 × 105 cells per lane of LCLs were loaded. LMP1 expression was detected by Western blot using the monoclonal antibody cocktail CS1-4 and HRP-conjugated secondary antibodies. LMP1 expression was detected in transduced fibroblasts as well as LCLs, and the level of LMP1 expression was higher in fibroblasts than in LCLs. Protein standards are indicated at the left in kilodaltons (*cross-reactive band only present in fibroblasts).
