LMP1-specific CTL lines recognize autologous LCLs.

The ability of T cells in PBMCs, EBV-specific CTLs, and LMP1-specific CTLs to secrete IFN-γ in response to autologous LCLs was determined in ELISPOT assays. Spot-forming cells (SFCs) were quantified by Zellnet Consulting. The frequency of LCL-reactive CTLs in LMP1-specific CTLs was 8.5% for donor A, 2.0% for donor B, and 4.6% for donor C and, in PBMCs, 0.34%, 0.57%, and 0.68%, respectively.