ΔLMP1 is functionally inactive in dendritic cells.

(A) Day 5 immature DCs were transduced with Ad_EGFP, Ad_ΔLMP1, and Ad_LMP1 at an MOI of 3. After 48 hours, cell-free media were collected and the concentration of TNF-α and IL-10 was determined by FACS using the Cytokine Cytometric Bead Array kit (BD Biosciences). Only LMP1 induced high levels of TNF-α and IL-10. (B) Day 5 immature DCs were transduced at an MOI of 6 and matured with a cytokine cocktail for 2 days prior to FACS analysis for DR, CD83, and ΔLMP1 expression (donors A, B, and C; n = 15). ΔLMP1 expression did not affect the expression of DR and CD83 in comparison with nontransduced DCs (control).