LMP1 expression is toxic in dendritic cells.

Day 5 immature DCs were transduced with increasing MOIs of Ad_LMP1 and Ad_ΔLMP1. After transduction, the DCs were matured with a cytokine cocktail for 2 days. (A) LMP1 expression and (B) the percentage of viable large cells were determined by FACS analysis. The transduction efficiency of Ad_LMP1 was lower than Ad_ΔLMP1, and LMP1 expression was toxic.