Distribution of VWF and fibrinogen in thrombi generated under a high shear rate.
Whole blood, without mepacrine labeling, from a healthy control or type 2B VWD patients (2B-3, -4) was perfused over a collagen surface at a high shear rate (1500 s−1 thrombi generated at 7 minutes of perfusion were fixed, double-stained with Cy2-labeled anti-VWF and Cy3-labeled antifibrinogen antibodies, and viewed by CLSM. The images displayed are cross-sections at a height of 15 μm from the collagen surface (original magnifications × 400) and are representative of 2 independent perfusions of blood from a control or patient, respectively. Unlike control thrombi, VWF is poorly stained (green, left panels), compared with fibrinogen (orange, right panels), in type 2B VWD thrombi.
