Effect of high levels of ectopic HOXB4 in human CD34⁺ cells on the number of hematopoietic progenitor cells.

High levels of ectopic HOXB4 in human CD34⁺ cells reduce the number of hematopoietic progenitor cells in vitro. This figure shows the frequency and distribution of colonies derived from CD34⁺ cells transduced with different HOXB4 vector contructs and the GFP control vector. Flow cytometrically sorted GFP⁺ cells (A) and unsorted transduced cells (B) for each vector were cultured in duplicate in methylcellulose-based media. Data are displayed as the mean ± standard error (bar) number of colonies per 500 CD34⁺ input cells. Statistical analysis was carried out by means of the Student t test (paired; 2-sided): *P < .05.