Fig. 5.
Fig. 5. Fluorescence images of CD47, lipid, and glycophorin C on normal and Rhnull-aspirated erythrocytes. / (A) FIMD of CD47-labeled cells (using TRITC-BRIC126 Fab) shows gradient from entrance to cap, indicative of considerable cytoskeletal attachment. (B) FIMD of CD47-labeled Rhnull cells (using TRITC-BRIC126 Fab) shows similar cytoskeletal attachment. (C) FIMD of lipid bilayer–labeled Rhnull cells illustrates the absence of any significant gradient, consistent with a homogeneous fluid bilayer. (D) FIMD of glycophorin C–labeled Rhnull cells illustrates the strong entrance to cap gradient, consistent with a cytoskeletally linked protein.

Fluorescence images of CD47, lipid, and glycophorin C on normal and Rhnull-aspirated erythrocytes.

(A) FIMD of CD47-labeled cells (using TRITC-BRIC126 Fab) shows gradient from entrance to cap, indicative of considerable cytoskeletal attachment. (B) FIMD of CD47-labeled Rhnull cells (using TRITC-BRIC126 Fab) shows similar cytoskeletal attachment. (C) FIMD of lipid bilayer–labeled Rhnull cells illustrates the absence of any significant gradient, consistent with a homogeneous fluid bilayer. (D) FIMD of glycophorin C–labeled Rhnull cells illustrates the strong entrance to cap gradient, consistent with a cytoskeletally linked protein.

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