Distribution of RV DNA in mice.

DNA was isolated from organs, and the number of copies of the RV per diploid genome was determined by real-time PCR and shown as the average ± SEM. Two nontransduced normal mice from each group had no detectable RV sequences (fewer than 0.002 copies per cell) in any organ (not shown). (A) Copy number after transduction of newborns. The RV DNA copy number was determined in the indicated organs at 10 months after neonatal transduction for 3 normal C57BL/6 mice. The average plasma cFIX levels for these animals was 5.9 ± 2.5 μg/mL (SEM). (B) Copy number after transduction of adult mice. The RV DNA copy number was determined at 11 months after transduction for the BALB/c mice, whose expression levels are shown in Figure 2B, that were transduced as adults with hAAT-cFIX–WPRE after preceding administration of HGF. Large and small intestines are labeled Lg Int and Sm Int, respectively.