HHV8 vFLIP activates the NF-κB pathway in TF-1 cells.

(A) Electrophoretic mobility shift assay demonstrating persistent NF-κB activation in TF-1 cells expressing vFLIP K13. The positions of the induced NF-κB complexes are marked with arrows while an asterisk (*) marks the position of a constitutive NF-κB complex. (B) Western blot analysis demonstrating increased phosphorylation (top panel) and decrease in the total IκBα protein (bottom panel) in TF-1 cells expressing vFLIP K13.