Cell analyses of representative normal samples before and after immunomagnetic bead enrichment.

2-color dot plots or histogram plot of FACS analysis of the tonsil mononuclear fractions prior to CD19 selection (A), tonsil mononuclear fractions prior to CD138 selection (C), and bone marrow mononuclear cells prior to CD138 selection (E). 2-color dot plots or histogram plot of dual antibody FACS analysis of cell surface phenotype, light microscopy of morphology, and immunofluoresence microscopy of cytoplasmic immunoglobulin light chain expression in CD19-enriched tonsil BCs (B), CD138-enriched tonsil PC (D), and CD138-enriched bone marrow PCs (F). Antibodies used in individual FACS analyses are indicated to the left and bottom of each FACS plot. The percentage of cells in each gate window is indicated. Original magnification, × 60. Stains are described in “Materials and methods.”